Three are dedicated to scienceMedium pressure preparative chromatographyThe research and development, production, and application services of Purifier200 high-pressure preparative chromatography are conducted. Purifier200 is an efficient and powerful modular preparative chromatography system that improves the purification process in traditional Chinese medicine, chemical synthesis, and biochemical protein drug separation. It allows for gradient elution using up to four different solvents and simultaneous online detection of two wavelengths,It can easily store and call methods, and complete fraction collection work on the same platform,The system supports the connection of stainless steel chromatography columns and high-pressure glass chromatography columns, and is widely used for the separation, purification, and preparation of target active ingredients such as Chinese herbal medicine, natural products, organic synthetic products, and protein biopolymers. From laboratory research to industrial production, it can meet their requirements.
Purifier 200Medium pressure preparative chromatographySystem technical features:
*Microprocessor control, high-speed dual drive and parallel pump headsHigh speed cavityRoom pressure feedback, compensation refilling, and solvent compression effects achieve high reproducibility flow rates over a wide dynamic range.
*Adopting cam curve compensation technology to effectively control flow pulsation and ensure low baseline noise.
*Multi point flow correction curve ensures flow accuracy throughout the entire flow range.
*Floating plunger design ensures the service life of high-pressure sealing rings.
*10 user programs that can implement flow and gradient programming.
*Dual wavelength detection, wavelength time program, and pump stop scan - three measurement methods reduce baseline noise and drift, achieving high sensitivity and low detection limit, as well as a wider linear range. Corresponding to various measurement requirements, reliable quantification of main components, by-products, and impurities can be carried out simultaneously.
*It can quickly and conveniently replace the circulation pool, ensuring maximum normal operation time.
Automatic Collector Features:
• Unique motion principle, combining linear and rotational motion, can quickly reach any collection position;
Multiple collection modes are available, including volume, time, threshold, and slope combinations, to meet various collection needs
Establish regular mode, sequential collection, and cyclic collection;
Small pipeline design to reduce inaccurate collection caused by sample diffusion in the flow cell;
• Setting software delay volume for accurate collection and purer product;
Adopting high-precision bottle cutting technology, the waste liquid channel is independent, and there is no dripping during the bottle switching process;
Divided into two collection methods, dynamic and automatic, with simple and convenient operation;
The supporting software can collect multiple wavelength signals in real time, and the collected signals can be selected arbitrarily;
Real time display of device status, connection, and collection bottle location, with intuitive collection and clear location;
• Compatible with multiple collection containers, allowing up to bottles to be collected: 120 13-15mm test tubes;
• It has a self identification function for collection containers, which can prevent misplacement when using different types of collection containers;
Maximize space utilization, with devices occupying minimal space and easy to use.
Purifier 200Medium pressure preparative chromatographyTechnical parameters:
Pump head |
316L stainless steel pump with high precision, low pulse, and corrosion resistance (Peek pump head optional) |
Flow velocity range |
0.01-200.00ml/min (gradient) |
Flow velocity accuracy |
±0.5% |
Pressure range |
0-5MPa |
Pressure pulsation |
≤0.2MPa |
Gradient type |
Steps, linearly varying gradients, adjustable gradients and flow velocities online |
Minimum gradient adjustment |
1% |
Detector light source |
Deuterium lamp+tungsten lamp (imported) |
Detection wavelength |
190-800nm full wavelength detector for dual wavelength simultaneous detection |
Wavelength accuracy |
±1nm |
Range of absorbance |
0-2AU |
Collect |
Fully automatic collector |
Collecting pipe racks |
2 × 60 test tubes (Φ 15mm * 150mm test tubes), other specifications can be optionally selected |
Collection mode |
Normal mode (collect by time, peak, threshold), sequential collection, cyclic collection |
Manual sample valve |
Prepare chromatography valve (standard 10ml quantitative ring) |
Sample loading method |
Solid or liquid sampling |
Power Supply |
220V±10% 50Hz |
Chromatography software control |
Control the setting and operation of components such as pumps, UV, and automatic collectors through Sanochrom chromatography software |
Control interface |
Graphical interface,USB interface+RS-232 can be connectedUsing a PC software workstation based on Windows 7/Windows 8/Windows 10,The software complies with the regulatory requirements of CFDA GXP and FDA 21CFR Part 11 |